目的 探讨用氯氨T法和Iodogen法进行适配子的¹³¹I标记的可行性及标记产物在不同条件下的稳定性。方法 用氯氨T法和Iodogen法分别对适配子进行¹³¹I的标记,用纸层析法测定2种方法不同反应时间的标记率,测定标记产物在不同条件下的稳定性。结果 2种方法在反应时间为1 min时的标记率分别为（94.11±0.30） %和（94.01±0.07） %,比较差异无统计学意义（P > 0.05）;置于4℃与室温24 h后,标记产物在磷酸盐缓冲液（PBS）、无血清培养基、含10%血清培养基、纯血清中的放射性化学纯度分别为（97.46±0.77） %、（94.76±1.57） %、（85.18±2.27） %、（67.96±1.91） %与（95.78±1.98） %、（56.11±4.06） %、（23.96±3.76） %、（9.45±1.75） %,除PBS介质外,标记产物在4℃条件与室温条件下的放射性化学纯度均有差异（P均 < 0.001）;2种温度条件下标记产物在无血清培养基与含10%血清培养基中的放射性化学纯度均有差异（P均 < 0.001）。结论 氯氨T法和Iodogen法均可高效标记适配子,标记产物在低温及无血清条件中稳定性更好。

Objective To investigate the feasibility of labeling aptamer with ¹³¹I using chloramine-T or Iodogen methods and evaluate its stability under different conditions.Methods Aptamer was labeled with ¹³¹I using the chloramine-T and Iodogen methods. The labeling rate of different reaction times were measured by paper chromatography. The stability of labeled products under different conditions was determined.Results The labeling rates of two methods at 1 min of reaction time were （94.11±0.30） % and （94.01±0.07） %, respectively, there was no significant difference between them （P > 0.05）. The radiochemical purity of labeled products in phosphate buffer（PBS）, serum-free medium, 10% serum medium and serum were （97.46±0.77） %, （94.76±1.57） %, （85.18±2.27） % and （67.96±1.91） % at 4℃for 24 h, （95.78 ±1.98） %, （56.11±4.06） %, （23.96±3.76） % and （9.45±1.75） % at room temperature for 24 h, respectively. Statistical significance was observed under two temperature conditions in serum-free medium, 10% serum medium and serum （all P < 0.001）. At 4℃ and room temperature, the radiochemical purity of labeled products in the serum-free medium and 10% serum medium significantly differed （both P < 0.001）.Conclusion Both chloramine-T and Iodogen methods can efficiently label the aptamer, and the labeling products have better stability under low temperature and serum-free conditions.