Objective To investigate the changes of some endogenetic opioids peptides and the expression of preprodynorphin(PPD) and preproenkephalin(PPE) gene in patients under intravenous combined with inhaling general anesthesia during operation. Methods Twenty cases of patients with ASAⅠ-Ⅱ were treated with intravenous propofol using target-controlled infusion(TCI) combined with inhaling isofluran. β-endorphin, Leucine-enkephalin and Dynorphin A were measured with radioimmunoassay at 0, 20, 40, 60, 80 min since the beginning of operation. Blood was taken at 0, 80 min and the expression of PPD and PPE genes of lymphocytes were tested with RT-PCR. Results The plasma levels of β-endorphin and LEK patients were significantly increased at 20, 40, 60, 80 min since the beginning of operation as compared with 0min, and Dynorphin A was increased at 40, 60 min since the beginning of opertation. The expression of PPD and PPE genes at 80 min since the beginning of operation is higher than that before the beginning of operation. Conclusion The expression of pre-opioids peptides genes increased in patients under intravenous combined with inhaling general anesthesia during operation.
