Abstract: Objective To investigate the molecular epidemiological characteristics of drug sensitivity phenotype, capsular serotyping andcarrying of drug resistance gene of Haemophilus influenzae. Methods Haemophilus influenzae strains （n = 107） were collected from January to October, 2018. The capsular serum antigen of Haemophilus influenzae was detected by PCR analysis. The drug resistance genes of clinical isolates were identified by PCR analysis. Results Among 107 Haemophilus influenzae strains, β-lactamase gene was identified in 72 strains, which was consistent with the results of Nitrocefin test. Seventy （65.4%） Haemophilus influenzae strains were positive for TEM gene, two （1.9%） positive for ROB gene, two （1.9%） positive for bexA and three （2.8%） positive for bexB, respectively. Only one strain could be typed into f group, whereas the other strains were non-typable Haemophilus influenzae （NTHi）. Ten strains （9.3%） wereβ-lactamase non-producing ampicillin resistant Haemophilus influenzae （BLNAR）. Conclusions NTHi strain is the dominant type of Haemophilus influenzae in Jinan, China. The resistance to ampicillin among Haemophilus influenzae strains due to production of β- lactamase and non-production of β- lactamase is both increased.