Abstract: Objective: To study the effect of male factors on the intrauterine insemination (IUI) pregnancy rate, and provide references for IUI. Methods: A retrospective analysis of 219 cycles were involved in the study. The IUI data of infertility due to female or both gender factors was excluded. Result: The overall pregnancy rate (PR) was 10.5% (23/219). The sperm morphology normal rate was decreased along with aging. And the PR of >40 year-old group was lower than that of younger groups. Highly educated group’s PR was also lower than other education groups. No statistical difference was found related to infertility duration. The PR of the sperm morphology normal rate group was higher in than in the abnormal group, and IUI PR was in a rising trend along with the rise of the total motile sperms after processed, but there was no statistically significant found in them. Conclusion: Male’s age affected negatively on sperm morphology, but no significant difference was found regarding male’s education, infertility duration, total advancing sperms and sperm morphology to IUI pregnancy rate.