Abstract: Objective：To investigate the effects of resistin on insulin signaling in human umbilical vein endothelial cells（HUVECs）. Methods：HUVECs were primary cultured，then treated with recombinant human resistin (0～100μg/L) for 24 h. The levels of phospho-protein kinase B(p-PKB) and phospho-endothelial nitric oxide synthase（p-eNOS）were measured by western blotting in HUVECs under insulin stimulated conditions. Results：Compared with control group without resistin， the levels of p-PKB and p-eNOS in HUVECs by treatment 24 h with resistin were downregulated under insulin stimulated condition (P<0.05～0.01). Under basal condition, the level of p-PKB in HUVECS by treatment with resistin was not reduced, whereas it showed a decrease of p-eNOS (P<0.05). Conclusion：Resistin exerts an inhibitory effect on the insulin signaling and eNOS phosphorylation in endothelial cells. It may be one of the mechanisms that resistin induces parafunction of endotheliocyte.