Abstract: Objective：To evaluate the effect and safety of 10-day sequential therapy consisted of pantoprazole, clarithromycin, amoxicillin and tinidazole, and 7-day triple therapy in eradicating Helicobacter pylori(Hp) infection. Methods：One hundred and five patients of peptic ulcer and chronic gastritis with Hp-positive were randomly divided into two groups. Ten-day sequential treatment group (53 cases) was pantoprazole 40 mg, amoxicillin 1 000 mg, orally two times a day in first five days, then pantoprazole 40 mg, clarithromycin 500 mg, tinidazole 500 mg, orally two times a day in the second five days. Seven-day triple treatment group (52 cases) was pantoprazole 40 mg，clarithromycin 500 mg and amoxicillin 1 000 mg, orally two times a day for seven days. Results：The eradication rate of 10-day sequential therapy was 90.6%, and the eradication rate of 7-day triple therapy was 71.2%，the result showed a significant difference (P<0.05). The incidence rates of adverse reaction in 10-day sequential therapy group (18.9%) and 7-day triple therapy group (17.3%) were similar (P>0.05). Conclusion：The 10-day sequential therapy including pantoprazole, amoxicillin, clarithromycin and tinidazole was more effective than 7-day triple therapy on eradication of Hp infection.