轻度热应激对脾脏巨噬细胞免疫功能的影响

摘要: 目的：探讨轻度热应激对脾脏巨噬细胞免疫功能的影响。方法：原代培养鼠脾脏巨噬细胞，将细胞置于41℃恒温箱中，使细胞轻度热应激，1 h后恢复到37℃，分别在应激后0 min、30 min、60 min、120 min、180 min检测巨噬细胞的吞噬功能、杀伤活性和趋化作用。结果：轻度热应激后，脾脏巨噬细胞的吞噬功能、杀伤活性和趋化作用均比应激前明显加强（P<0.05～0.01），60 min后到达高峰，随后逐渐下降。结论：轻度热应激对脾脏巨噬细胞的免疫功能有促进作用。

Abstract: Objective：To investigate the effect of mild heat stress on immunological function of splenic macrophage. Methods：Macrophages were isolated from rat spleen and primarily cultured. Mild heat stress was performed by incubating cells in a 41 ℃ thermostat container for 1 h and recovering to 37 ℃ thereafter. Phagocytosis， cytotoxicity， and chemotaxis functions of macrophages were evaluated at 0 min， 30 min， 60 min， 120 min and 180 min after mild heat stress，respectively. Results： Phagocytosis， cytotoxicity， and chemotaxis functions of macrophage were enhanced after mild heat stress（P<0.05～0.01）. The maximal effect occurred after 60 min and then gradually decrased. Conclusion：Mild heat stress promotes the immunological function of splenic macrophage.