Abstract: Objective：To evaluate the relationship between volatile organic compound(VOC) in incubator and embryo quality, pregnant outcome during in vitro fertilization-embryo transfer(IVF-ET). Methods One hundred and five cycles of 100 patients from September to October 2010 were selected. The VOC level in incubator was measured in the morning on D1, D2, D3 after egg retrieval. Seventy cycles of patients received transfer were divided into two groups according to whether they were pregnant or not. The relationship between VOC level in incubator and embryo quality, pregnant outcome were analyzed. Results There is no significant difference in VOC level among D1, D2, D3. It showed no significant correlation not only between D1 VOC level and fertilization rate, but also between total VOC level and rate of Oocytes cleavage, quality of embryo, implantation rate of embryo transfer(P>0.05). The age, cycles, basic follicle-stimulating hormone level, obtainment of mature oocytes, fertilized number, transferred embryos, D1 VOC level, D2 VOC level, D3 VOC level, total VOC level and average VOC level had no significant difference between pregnant group and non-pregnant group(P>0.05). Conclusions The VOC level which were detected in this study will not harm embryo quality and pregnant outcome. The incubators used in our center are safe to cultivate embryos in VOC part.