Abstract: Objective To evaluate the efficacy and safety of linezolid in the treatment of neonatal purulent meningitis. Methods Clinical data of 14 suppurative meningitis children treated with linezolid alone or combined use of linezolid were retrospectively collected and analyzed.According to the symptoms, physical signs, cerebrospinal fluid examination and bacterial culture results before and after linezolid treatment, clinical efficacy and safety of linezolid in the treatment of neonatal purulent meningitis were evaluated. Results Cerebrospinal fluid culture confirmed that 14 cases were infected with Gram-positive coccal bacteria or suspectedly complicated with Gram-positive coccal bacterial infection after preliminary treatment.The total effective rate was 92.9% （13/14） after linezolid therapy.After corresponding treatment, body temperature, total amount of cerebrospinal fluid cells, leukocyte count and the protein level in cerebrospinal fluid were significantly lower, whereas the glucose level in cerebrospinal fluid was significantly higher than those before treatment （all P < 0.05）.The leukocyte count was declined in 2 children after treatment.No linezolid-induced adverse events occurred in 12 cases. Conclusion Linezolid can effectively and safely treat Gram-positive coccal bacteria-induced purulent meningitis in neonates.