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外周血pNF-H、S100B水平与脊柱骨折伴脊髓损伤患者病情程度的相关性
Correlation between the expression of pNF-H and S100B in peripheral blood and the severity of spinal fracture complicated with spinal cord injury
目的 探讨外周血神经丝蛋白H磷酸化亚型（pNF-H）、S100B钙结合蛋白（S100B）水平与脊柱骨折伴脊髓损伤患者病情程度的相关性。 方法 选择238例脊柱骨折患者,根据其是否伴脊髓损伤分为脊髓损伤组71例与脊髓未损伤组167例,采用ELISA检测血清pNF-H、S100B水平,比较2组血清pNF-H、S100B水平,对比病情不同严重程度的脊柱骨折伴脊髓损伤患者血清pNF-H、S100B水平,绘制受试者工作特征（ROC）曲线分析血清pNF-H、S100B水平对脊柱骨折伴脊髓损伤的诊断价值。 结果 脊髓损伤组血清pNF-H、S100B水平高于脊髓未损伤组（P均< 0.05）。脊髓完全损伤的脊柱骨折患者血清pNF-H、S100B水平高于脊髓不完全损伤的脊柱骨折患者（P均< 0.05）。血清pNF-H、S100B水平联合检测对脊柱骨折伴脊髓损伤的ROC曲线下面积均高于单独检测（P均< 0.05）。 结论 pNF-H、S100B水平在脊柱骨折伴脊髓损伤患者外周血中高表达,并与脊柱骨折伴脊髓损伤患者病情严重程度相关,检测血清pNF-H、S100B水平有助于脊柱骨折伴脊髓损伤早期诊断及病情严重程度评估。
Objective To investigate the correlation between the expression levels of phosphorylated heavy-chain neurofilament （pNF-H） and S100B in the peripheral blood and the severity of spinal fracture complicated with spinal cord injury. Methods A total of 238 patients with spinal fractures were enrolled and divided into the spinal cord injury group （n = 71） and non-spinal cord injury group （n = 167） according to whether they were complicated with spinal cord injury. The serum pNF-H and S100B levels were quantitatively measured by enzyme-linked immunosorbent assay （ELISA） and statistically compared between two groups. In addition, the serum pNF-H and S100B levels were statistically compared among patients with varying severity of diseases. The receiver operating characteristic （ROC） curve was delineated to evaluate the diagnotic values of serum pNF-H and S100B levels for spinal fracture complicated with spinal cord injury. Results Serum pNF-H and S100B levels in the spinal cord injury group were significantly higher than those in the non-spinal cord injury group （both P < 0.05）. Serum pNF-H and S100B levels in patients with complete spinal cord injury were significantly higher than those in their counterparts with incomplete spinal cord injury （both P < 0.05）. The area under ROC curve （AUC） of combined detection of serum pNF-H and S100B for spinal fracture complicated with spinal cord injury was significantly higher than that of single detection （both P < 0.05）. Conclusions Both pNF-H and S100B are highly expressed in the peripheral blood of patients with spinal fracture complicated with spinal cord injury, which are significantly correlated with the severity of the diseases. The detection of two biomarkers contributes to early diagnosis and severity assessment of spinal fracture complicated with spinal cord injury.
神经丝蛋白H磷酸化亚型 / S100B钙结合蛋白 / 脊柱骨折 / 脊髓损伤 / 表达水平 / 病情严重程度 / 相关性 {{custom_keyword}} /
Phosphorylated heavy-chain neurofilament / S100B / Spinal fracture / Spinal cord injury / Expression level / Disease severity / Correlation {{custom_keyword}} /
表1 脊髓损伤组与脊髓未损伤组血清pNF-H、S100B水平比较（ |
| 组 别 | 例数 | pNF-H | S100B |
|---|---|---|---|
| 脊髓损伤组 | 71 | 0.49±0.15 | 0.55±0.13 |
| 脊髓未损伤组 | 167 | 0.12±0.05 | 0.32±0.06 |
| t值 | 28.440 | 18.690 | |
| P值 | < 0.001 | < 0.001 |
表2 脊髓完全损伤与不完全损伤的脊柱骨折患者血清pNF-H、S100B水平比较（ |
| 病情严重程度 | 例数 | pNF-H | S100B |
|---|---|---|---|
| 脊髓完全损伤 | 24 | 0.69±0.21 | 0.76±0.19 |
| 脊髓不完全损伤 | 47 | 0.38±0.12 | 0.44±0.10 |
| t值 | 7.927 | 9.327 | |
| P值 | < 0.001 | < 0.001 |
表3 血清pNF-H、S100B水平对脊柱骨折伴脊髓损伤的诊断价值 |
| 指 标 | 灵敏度 | 特异度 | 阳性预测值 | 阴性预测值 | 准确度 | 截断值 | AUC | 95%CI |
|---|---|---|---|---|---|---|---|---|
| pNF-H | 0.775 | 0.851 | 0.683 | 0.609 | 0.574 | 0.26 | 0.825 | 0.762 ~ 0.888 |
| S100B | 0.692 | 0.563 | 0.677 | 0.871 | 0.683 | 0.40 | 0.778 | 0.710 ~ 0.847 |
| pNF-H+S100B | 0.932 | 0.867 | 0.919 | 0.912 | 0.917 | / | 0.904 | 0.863 ~ 0.946 |
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辛坤, 王婷婷. 胸腰段脊柱骨折合并脊髓损伤者术后发生下肢深静脉血栓的危险因素分析. 颈腰痛杂志, 2019,40(3):431-432.
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S100B is one of the members of the S100 protein family and is involved in the progression of a variety of cancers. Ovarian cancer is driven by cancer stem-like cells (CSLCs) that are involved in tumorigenesis, metastasis, chemo-resistance and relapse. We then hypothesized that S100B might exert pro-tumor effects by regulating ovarian CSLCs stemness, a key characteristic of CSLCs. First, we observed the high expression of S100B in ovarian cancer specimens when compared to that in normal ovary. The S100B upregulation associated with more advanced tumor stages, poorer differentiation and poorer survival. In addition, elevated S100B expression correlated with increased expression of stem cell markers including CD133, Nanog and Oct4. Then, we found that S100B was preferentially expressed in CD133(+) ovarian CSLCs derived from both ovarian cancer cell lines and primary tumors of patients. More importantly, we revealed that S100B knockdown suppressed the in vitro self-renewal and in vivo tumorigenicity of ovarian CSLCs and decreased their expression of stem cell markers. S100B ectopic expression endowed non-CSLCs with stemness, which has been demonstrated with both in vitro and in vivo experiments. Mechanically, we demonstrated that the underlying mechanism of S100B-mediated effects on CSLCs stemness was not dependent on its binding with a receptor for advanced glycation end products (RAGE), but might be through intracellular regulation, through the inhibition of p53 expression and phosphorylation. In conclusion, our results elucidate the importance of S100B in maintenance of ovarian CSLCs stemness, which might provide a promising therapeutic target for ovarian cancer. Stem Cells 2017;35:325-336.
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The current study aimed at the investigating the potential use of phosphorylated neurofilament H (pNF-H) as a diagnostic biomarker for neurologic disorders in the horse. Paired serum and cerebrospinal fluid (CSF) samples (n=88) and serum only (n=30) were obtained from horses diagnosed with neurologic disorders and clinically healthy horses as control. The neurologic horses consisted of equine protozoal myeloencephalitis (EPM) (38 cases) and cervical vertebral malformation (CVM) (23 cases). Levels of pNF-H were determined using an ELISA. The correlation between CSF and serum concentrations of pNF-H was evaluated using Spearman's Rank test and the significance of the difference among the groups was assessed using a nonparametric test. Horses had higher pNF-H levels in the CSF than serum. Horses afflicted with EPM had significantly higher serum pNF-H levels in comparison to controls or CVM cases. The correlation between CSF and serum pNF-H levels was poor in both the whole study population and among subgroups of horses included in the study. There was significant association between the likelihood of EPM and the concentrations of pNF-H in either the serum or CSF. These data suggest that pNF-H could be detected in serum and CSF samples from neurologic and control horses. This study demonstrated that pNF-H levels in serum and CSF have the potential to provide objective information to help in the early diagnosis of horses afflicted with neurologic disorders.
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陈锦景, 张旸, 朱春然, 余万. 血清S100B和NSE联合检测对颅脑损伤患者预后判断的价值. 现代医学, 2016,9(11):122-124.
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Ischemic stroke is the leading cause of worldwide mortality and long-term disability in adults. This study aims to explore the effects of RNA interference (RNAi)-mediated silencing of the S100B gene on nerve function recovery and morphological changes of hippocampus cells in rat models with ischemic stroke. Sixty Wistar rats were assigned into different group. S100B and Caspase 3 mRNA and protein expressions were evaluated by RT-qPCR and Western blotting. Positive rate of S100B, NeuN, and MAP2 expressions were detected by immunohistochemistry (IHC). Water content, malondialdehyde (MDA) levels, and superoxide dismutase (SOD) activity in brain tissues were measured. Enzyme-linked immunosorbent assay (ELISA) was employed to detect serum levels of TNF-alpha and IL-1beta. A neurological severity score (NSS) was used to test nerve function. TUNEL assay was used to determine hippocampal cell apoptosis. Downregulation of S100B showed a lower number of S100B immune positive cells, but higher NeuN and MAP2-positive cells, increased SOD level, declined MDA level, prominently faster recovery of neurological function, decreased TRCS, TCTP, TCFP, and IE levels, an obvious increase in the number of survival neurons, a decrease in the number of apoptotic cells, notably decreased TNF-alpha and IL-1beta contents, as well as infarct volume, an obvious decrease in positive hippocampal cell Caspase 3 expression and protein expressions of Caspase 3 and cleaved Caspase 3. This study provides data to suggest that RNAi-mediated silencing of S100B gene could improve the recovery of nerve function while inhibiting apoptosis of hippocampal cells in rats with ischemic stroke.
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Chemoresistance is one of the most important causes of ovarian cancerrelated deaths. Recently, cancer stem cells (CSCs) have been recognized as the source of chemoresistance in ovarian cancer. However, the underlying mechanisms that regulate the chemoresistance of ovarian CSCs (OCSCs) remain unclear. The aim of the present study was to investigate the roles of S100B in the regulation of OCSC chemoresistance, which provides a novel therapeutic target. We observed high expression of S100B in CD133+ OCSCs derived from ovarian cancer cell lines and primary tumors and in cisplatinresistant patient samples. Then, we determined that S100B knockdown promoted the apoptosis of OCSCs after treatment with different concentrations of cisplatin. The underlying mechanism of S100Bmediated chemoresistance in OCSCs may be through p53 inhibition. Furthermore, drugresistance genes, including MDR1 and MRP1, were involved in the process of S100Bmediated OCSC chemoresistance. In conclusion, our results elucidated the importance of S100B in the maintenance of OCSC chemoresistance, which may provide a promising therapeutic target for ovarian cancer.
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张振雨. 脊柱骨折伴急性脊髓损伤患者血清NSE、S100B蛋白水平变化及意义. 颈腰痛杂志, 2019,20(3):336-339.
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PDF(1057 KB)
表1 脊髓损伤组与脊髓未损伤组血清pNF-H、S100B水平比较（表2 脊髓完全损伤与不完全损伤的脊柱骨折患者血清pNF-H、S100B水平比较（
图1 血清pNF-H、S100B诊断脊柱骨折伴脊髓损伤的ROC曲线表3 血清pNF-H、S100B水平对脊柱骨折伴脊髓损伤的诊断价值/
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