Objective：To investigate the effects of ginkgetin aglycone(GA) on the expression of P-selectin and lectin-like oxidized low density lipoprotein receptor-1（LOX-1）induced by oxidized-low density lipoprotein （ox-LDL） in human umbilical venous endothelial cells. Methods：Vessel endothelial cells 304(ECV304) were cultured ，then the cells were divided into control group, ox-LDL-treated group, combining LOX-1 receptor blocker polyinosinic acid(PIA)-stimulated and ox-LDL-treated group，combining GA-stimulated (6.25 mg/L、12.5 mg/L、25 mg/L and 50 mg/L) and ox-LDL-treated group. RT-PCR was used to detect the expression of P-selectin mRNA and LOX-1 mRNA. P-selectin protein was measured by ELISA. LOX-1 protein level was analyzed by SP immunohistochemistry assay. Results：ox-LDL treatment markedly increased the expression of P-selectin and LOX-1 in the ECV304 cells (P<0.05) , and 6.25～50 mg/L GA could inhibit the expression of P-selectin and LOX-1 induced by ox-LDL significantly(P<0.05). PIA partly or completely inhibited the expression of P-selectin mRNA， LOX-1 mRNA and its protein induced by ox-LDL in the endothelial cells (P<0.05). Conclusions: GA suppresses the secretion of adhesion molecule P-selectin by inhibiting the expression of LOX-1 in endothelial cell, and it may be one of the mechanisms of anti-atherosclerosis.
