Objective：To discuss the feasibility of using real-time quantitative PCR technique on HBV DNA screening for corneal graft material. Mehtods：The blood and extraocular fascia, cornea, pigmented membrane of 22 cases of corneal transplantation donor were qualitatively and quantitatively analyzed with real-time quantitative PCR. The specificity and sensitivity of blood specimen and ocular tissues were compared in detecting HBV DNA, and the differences of HBV DNA content among ocular tissues were done. Results：In 18 patients with HBV positive and 4 cases of HBV negative donor material, sensitivity are respectively 83%（15/18）, 100%（18/18）, 83%（15/18） and 78%（14/18） in blood, extraocular fascia, cornea and pigment membrane HBV DNA detection, and the specificity were all 100%. Extraocular fascia, corneal and pigmented membrane HBV DNA quantitative analysis showed, unit quality pigment membrane HBV DNA load is highest, extraocular fascia and corneal HBV DNA load no statistical difference. Conclusion：Application of real-time quantitative PCR technique for the detection of HBV DNA in extraocular fascia can be used as a corneal transplant donor pathogen screening detection means.
